Fig. 3

Evolutionary overview of GP5 whole genome sequenced samples. a Subclonal reconstruction of single nucleotide variants (SNVs) across the cancer samples (rows) into evolutionary clusters (colored segments in columns). Vertical lines on sample rows represent SNVs with the height of the lines corresponding to their cancer cell fractions (CCF) in the samples, mirrored on the negative side of the axis. Curve-fitted median cluster CCFs are shown on top of each cluster in every sample. The mutations in the most recent common ancestor (MRCA) truncal cluster T are present with a ~ 1.0 CCF in every sample, signifying that these mutations are present in every detected cancer cell. Subclonal SNVs are present with lower CCFs in the samples where the subclone is detected. Mutations detected only in a single sample (private mutations) appear in gray in the rightmost columns. The number of SNVs in each cluster is shown at the bottom of the columns. b Cladogram of the evolutionary clusters in GP5 cancer. The MRCA, represented by Cluster T, branched into two distinct lineages, a non-metastatic A-branch (green) and a metastatic B-branch (orange). c “Jawbreaker” plots combine evolutionary cluster information with the cluster CCFs to create a summary of the fractions of cancer cell populations present in each sampled location at the time of radical prostatectomy. Example jawbreaker plot at the top in gray describes how descending subclonal lineages are drawn on top of each other so that the surface (outermost layer) of the jawbreaker matches the fraction of cells belonging to the color-matching evolutionary clusters (see also Additional file 1: Fig. S6 for jawbreaker interpretation)