Fig. 1
From: Acute ischemia induces spatially and transcriptionally distinct microglial subclusters
Identification of cell types. A Scheme of scRNA-seq. B t-SNE plot showing cell clusters in sham and MCAO (3-h, 12-h, and 72-h post-ischemic stroke) mouse brains. Colors highlight 15 transcriptionally distinct clusters. Cell types were manually annotated based on canonical marker gene expression (Additional file 2: Table S2). C Heatmap of top 10 marker genes enriched in each cell type based on bimod inspection. Genes of interest are highlighted. Cluster annotations and coloring are consistent across panels. D Proportional histogram depicting the proportion alterations of 15 transcriptionally distinct cell types at the acute stage of ischemic stroke (Sham, MCAO-3h, MCAO-12h, and MCAO-72h)