Fig. 2

Conjugation assays of gene-edited plasmids. a Alignment of the oriTs of pKPHS2 (p2), pKPHS3 (p3), and pRJF293 (pV). b Schematic diagram of conjugation assays for pKPHS2, pKPHS3, pRJF293 and their nonconjugative derivatives. The green square denotes the tra gene cluster on pKPHS2 (p2). The green square with white slashes denotes the incomplete tra gene cluster on pKPHS3 (pV). The red inverted triangle presents the deletion of the oriT on plasmids. The conjugative frequencies of detected transconjugants that acquired either pKPHS2 (p2) alone or both pKPHS2 (p2) and pRJF293 (p3) are listed. Detailed data are available in Additional file 6: Table S5