Fig. 3
Schematic diagram of the simulation of mobilization of the virulence plasmid pRJF293 (pV) and the MDR plasmid pKPHS3 (p3) with their oriTs and the help of the CR plasmid pKPHS2 (p2). a When accompanied by pKPHS2, pACYC184-oriTp2 can be transferred while the empty vector pACYC184 cannot. b When accompanied by plasmid pKPHS2, pACYC184-oriTp3 and pACYC184-oriTpV can be transferred. c pACYC184-oriTp2, pACYC184-oriTp3, and pACYC184-oriTpV cannot be transferred when accompanied by plasmid pKPHS2ΔtraI. d pACYC184-oriTp3 and pACYC184-oriTpV cannot be transferred alone. The orange circle denotes the backbone of plasmid pACYC184-Apr, and the blue circle denotes the conjugative plasmid pKPHS2. The green square denotes the tra gene cluster. The blue square denotes the oriT derived from plasmid pKPHS2. The purple square denotes the oriT derived from pKPHS3. The red square denotes the oriT derived from pRJF293. The conjugative frequencies of detected transconjugants that acquired either pKPHS2 (p2) or both pKPHS2 (p2) and pACYC184 derivatives are listed. Detailed data are available in Additional file 6: Table S5