Fig. 4

Accurate detection of cancer based on nucleosome occupancy and fuzziness. A A schematic diagram showing the differences between nucleosome occupancy and fuzziness for four example positions in four cells. B Genomic regions showing nucleosome occupancy (left panel) and fuzziness changes (right panel) between cancer and non-cancer samples. The top panel shows genome browser tracks of TSS target regions, and the bottom panel shows PAS target regions. For each panel, two example cancer and non-cancer samples are displayed. The blue boxes show the gene bodies with white arrows indicating the transcription directions. C, D ROC curves showing the model performances based on the nucleosome occupancy of TSS target regions (Occupancy TSS), PAS target regions (Occupancy PAS), or combination of the two (Occupancy TSS + PAS). E, F ROC curves showing the model performances based on nucleosome fuzziness of TSS target regions (Fuzziness TSS), PAS target regions (Fuzziness PAS), or combination of the two (Fuzziness TSS + PAS). PAS, polyadenylation site. For the ROC curves, results from 130 iterations for cohort 1 and 332 iterations for cohort 2 of leave-one-out cross-validation analysis were shown