Fig. 5

Regulatory T cells play an immunosuppressive role in TME. A Differentially expressed genes (DEGs) in subgroup analysis. Significant P values were labeled in red, and the y-axis represents the log2 fold change. The top 10 DEGs were denoted. B GO pathway enrichment analysis for baseline ESCC tumor in pCR patients compared to IPR patients. The x-axis represents the ratio of mRNAs enriched in GO terms. The y-axis represents the enriched pathway. The color and size of each bubble represent enrichment significance and the number of related mRNAs enriched in the pathway, respectively. C, D Dot plots illustrate the top 30 ligand-receptor interactions from Treg cells obtained from baseline ESCC tumors of IPR (C) and pCR patients (D). The size of the circle represents the P values, and the color of the circle indicates the average expression level of interacting pairs. The cell clusters labeled in blue and red on the x-axis indicate that Treg cells act as ligands and receptors in the interaction pairs, respectively. E, F Dot plots show the immune checkpoint ligand-receptor interactions from Treg cells obtained from baseline ESCC tumors of IPR (E) and pCR patients (F)