Open Access

Erratum to: Illuminating uveitis: metagenomic deep sequencing identifies common and rare pathogens

  • Thuy Doan1, 2,
  • Michael R. Wilson3, 4,
  • Emily D. Crawford3, 5,
  • Eric D. Chow3,
  • Lillian M. Khan3,
  • Kristeene A. Knopp3,
  • Brian D. O’Donovan3,
  • Dongxiang Xia6,
  • Jill K. Hacker6,
  • Jay M. Stewart2,
  • John A. Gonzales1, 2,
  • Nisha R. Acharya1, 2 and
  • Joseph L. DeRisi3Email author
Contributed equally
Genome Medicine20168:123

Received: 7 November 2016

Accepted: 7 November 2016

Published: 22 November 2016

The original article was published in Genome Medicine 2016 8:90


It has come to our attention that there is an error in Fig. 3a for this article [1]. The correct version of Fig. 3a can be found below. The red markers now reflect the sequence differences. The text is correct. There was also a row omitted in Additional file 1: Table S1. The revised version can be found below.
Fig. 3

Identification of rubella virus (RV) by metagenomic deep sequencing (MDS). a Illustrates how the 9688 nucleotide paired-end sequence reads obtained from sequencing the RNA extracted from subject 6’s aqueous fluid aligned to the most closely matched RV genome (GenBank DQ388280.1): 99.3% of the total RV genome is represented. Positions of synonymous (black vertical lines) and non-synonymous (red vertical lines) variants are shown. Of the 149 substitutions, 107 were synonymous and 42 were non-synonymous. Of the 42 non-synonymous mutations, 25 occurred within the coding region for the E1 and E2 glycoproteins. Per unit length, the number of non-synonymous mutations in the E1 and E2 proteins was 6.3-fold higher than in the non-structural proteins. The cyan marker above the E1 gene represents the 739-nucleotide sequence window recommended by the World Health Organization (WHO) for RV genotyping. b Phylogenetic analysis of subject 6’s RV strain obtained from MDS with 32 WHO reference strains, GUZ_GER92 (Stuttgart strain), and the RV27/3 vaccine strain, demonstrating that the subject’s RV sequence was most closely related to the genotype 1G viruses and not the vaccine strain



Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

Francis I. Proctor Foundation, University of California San Francisco
Department of Ophthalmology, University of California San Francisco
Department of Biochemistry and Biophysics, University of California San Francisco
Department of Neurology, University of California San Francisco
Howard Hughes Medical Institute
California Department of Public Health


  1. Doan T, Wilson MR, Crawford ED, Chow ED, Khan LM, Knopp KA, O’Donovan BD, Xia D, Hacker JK, Stewart JM, Gonzales JA, Archarya NR, DeRisi JL. Illuminating uveitis: metagenomic deep sequencing identifies common and rare pathogens. Genome Med. 2016;8:106.View ArticleGoogle Scholar


© The Author(s). 2016