Fig. 1

Overview of the present study design and establishment of transgenic flies co-expressing TREM2 with TYROBP. a Transgenic flies expressing or co-expressing human TREM2/TYROBP in glial cells, Aβ42 in neurons, or tau in the retina were developed. These fly models were characterized for several phenotypic changes. b RNA from control and transgenic fly heads were profiled by RNA-seq, to identify differentially expressed genes (DEGs). The gene ontology (GO) and pathway terms enriched for DEGs were identified, and several DEGs were validated by qPCR. c Two WGCNA (Weighted Gene Co-expression Network Analysis) co-expression networks and a Bayesian regulatory network (BN) were collected from gene expression datasets of two human AD cohorts (HBTRC and ROSMAP). Human orthologs of the fly DEGs were projected onto gene co-expression networks of human AD datasets to explore the relevance of these gene signatures to AD pathogenesis from a network prospective