Fig. 6

Glial expression of TREM2/TYROBP exacerbated tau-mediated neurodegeneration without altering tau phosphorylation levels. a Top: The lamina of control flies carrying drivers alone (control), flies with glial expression of TREM2^WT/TYROBP alone (TREM2^WT/TYROBP), flies with glial expression of TREM2^R47H/TYROBP alone (TREM2^R47H/TYROBP), flies expressing tau alone (tau), expression of tau and glial expression of TREM2^WT/TYROBP (Tau/TREM2^WT/TYROBP), and expression of tau and glial expression of TREM2^R47H/TYROBP (tau/TREM2^R47H/TYROBP). Flies are 1 day or 1 week after eclosion. Bottom: Quantification of neurodegeneration. Mean ± SEM, n = 12–18 hemispheres. ***p < 0.001, Student’s t-test. b Western blot analysis of fly head lysates with antibody against total tau and an antibody that recognizes tau without phosphorylation at Ser194, 195, 198, and 202 (non-phospho Tau), tau phosphorylated at Thr231 (pThr231 Tau), and tau phosphorylated at Ser262 (pSer262 Tau). Tubulin was used as a loading control. Representative blots are shown in the left and quantification in the right. Mean ± SEM, n = 4. Genotypes of flies are described in Additional file 2: Table S1